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KMID : 0043320170400010049
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Archives of Pharmacal Research
2017 Volume.40 No. 1 p.49 ~ p.56
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A simple and reliable analytical method based on HPLC?UV to determine oleanonic acid content in Chios gum mastic for quality control
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Jin Yan
Zhao Jing
Jeong Kyung-Min
Yoo Da-Eun
Han Se-Young
Choi Su-Young
Ko Dong-Hyeon
Kim Hyun-Ji
Sung Na-Hye
Lee Jeong-Mi
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Abstract
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A reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of oleanonic acid (OA) content in Chios gum mastic (CGM). A simple method involving methanol extraction of CGM powder followed by basification and ether extraction was developed to isolate the triterpenic fraction including OA. The triterpenic fraction was chromatographed on a Phenomenex Gemini C18 column (150 × 4.6 mm, 5 μm) under a simple gradient elution of a mobile phase containing acetonitrile and water at a flow rate of 1.0 mL min?1. The detection wavelength was set at 210 nm. Good linearity was achieved in the range of 100.0?1000.0 μg mL?1 with r2 > 0.9993, and the limit of quantification was 32.22 μg mL?1. Accuracy measured at three concentration levels was in the range of 93.72?99.56%, while intra-day and inter-day precisions estimated using both OA standard and CGM samples were no more than 2.83 and 4.57% RSD, respectively. Finally, this method was applied to real CGM samples from various batches, revealing that the OA contents were between 88.13 and 100.83 μg mg?1. These results suggest that the current method can be applied as an efficient analytical method for quality control of CGM.
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KEYWORD
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Chios gum mastic, Oleanonic acid, Method validation, Quality control
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